Speakers and Academics
Georg-August Universität Göttingen
My lab has a dual focus – the development of high-end imaging and synaptic physiology. Our projects combine super-resolution imaging techniques (STED, STORM, expansion microscopy) with conventional imaging, electron microscopy and quantitative biochemistry.
We provided the first quantitative molecular description of a part of the cell, the synapse (now included in the standard textbook for cellular and molecular biology, the “Molecular Biology of the Cell” by Alberts and colleagues, Garland Science, sixth edition). We currently extend our studies in the temporal direction by imaging cellular turnover in situ, at the nanoscale, using secondary ion mass spectrometry (SIMS).
Finally, we also focus on developing new imaging tools, ranging from new chemical probes to camelid-derived single-chain antibodies (nanobodies), which result in the improved visualization of cellular targets, especially at the nanoscale.
KTH Royal Institute of Technology, Stockholm
I am a cellular biologist in the lab of Ilaria Testa with an interest in neuroscience and optical microscopy.
My focus is to investigate the intricate network of mitochondria, their nanoscale organization and their dynamic distribution in relation to other organelles inside the complex neuronal morphology. Therefore, I am combining different optical methods with new labelling strategies to expand the possibilities for live-cell optical fluorescence nanoscopy of organelles in neurons.
University of Oxford
As an undergraduate at the University of Edinburgh, I studied Medical Microbiology and Infection before completing an interdisciplinary PhD in Electrical Engineering on the subject of Microfluidics for Non-Invasive Prenatal Diagnostics. During my postdocs, I applied advanced fluorescence microscopy and spectroscopy to study the molecular mechanisms underlying cell communication and trafficking.
Over the past four years, I have worked as an Imaging Specialist at University of Birmingham, where my primary role was in the teaching and training of researchers in advanced microscopy. In May 2021, I joined the University of Oxford as Microscopy Facility Manager for the Biochemistry Department where I am responsible for the running of a busy microscopy facility and continue my passion for microscopy teaching and training. I have expertise in fluorescence microscopy with specialist interest in super-resolution, FCS and light-sheet technologies.
University of Oxford
My research aims at understanding the relationship between 3D nuclear organisation and genome activity in mammalian cells by combining genetic tools and innovative live-cell and correlative super-resolution imaging and analysis approaches. This will ultimately enable to directly observe genome activity, such as transcription, replication and repair, in the context of the nuclear environment at the nanoscale.
As affiliated member of the Micron Advanced Bioimaging Unit, I am driving the development of computational analysis and fluorescence labelling tools for super-resolution microscopy.
King's College London
I am a Royal Society University Research Fellow in the Randall Division of Biophysics at King's College London.
Following a PhD in transmission electron microscopy at Cambridge, I spent three years at the National High Magnetic Field Laboratory at Los Alamos looking at the behaviour of the low temperature phases of strongly correlated electron systems.
My current primary interest is the development of new super-resolution localisation microscopy techniques, both through the development of optical systems and the creation of novel image analysis algorithms. I use these techniques to investigate the behaviour of the cytoskeleton in live cells at the nanoscale. In 2015, I was awarded the RMS Medal for Light Microscopy and the President's Medal of the Society of Experimental Biology for the Cell Section.
Sebastian van de Linde
University of Strathclyde
I am currently a Lecturer and Chancellor’s Fellow in the Department of Physics at the University of Strathclyde. In 2011, I obtained a PhD in Physics under the supervision of Markus Sauer at Bielefeld University, Germany, and later worked in the Department of Biotechnology and Biophysics, University of Würzburg, Germany.
It is already 13 years ago, when we developed dSTORM and started to explore the opportunities of SMLM; since then I worked extensively on the photophysics of organic dyes.
My group is working on the development of refined single-molecule imaging tools for super-resolution microscopy to study cellular life, including novel photoswitches, optical imaging schemes and software for image processing and data analysis. A special focus of my research interest is the quantitative imaging of membrane receptors.
Instituto Gulbenkian de Ciência
My background is in semiconductor physics and nanofabrication, but I switched to the Biophysics field for my Masters project at BCUBE in Dresden, combining biomolecular motors and quantum dots to probe optical nearfields at ultra-high-resolution. For my PhD, I joined the labs of Katrin Heinze and Markus Sauer in Würzburg, where I designed optimized metal-dielectric nanocoatings to enhance performance of super-resolution microscopy techniques. In 2021 I moved to Portugal to join Ricardo Henriques at the IGC, where I’m pushing super-resolution techniques towards open-source and live cell microscopy to study of host pathogen interactions. My work focuses on the development of machine-learning guided smart microscopy approaches to observe the mechanistic pathways of virus entry.
The Imaging and Modeling Unit of Institut Pasteur, develops computational and experimental approaches to characterize and quantitatively predict selected cellular processes.
Our current projects concentrate on : (i) investigating the dynamic spatial architecture of the genome and its functional consequences, and (ii) developing high resolution or high throughput imaging techniques, and applying them to study genome architecture and the cell biology of pathogens, especially HIV.
Our lab mobilizes a spectrum of expertise including biophysics, microscopy, informatics and cell biology, and works in close collaboration with several experimental groups, many of them at Institut Pasteur.
Industry Partner Workshops
Digital Imaging with low-noise Cameras
Spinning disk super resolution by optical pixel reassignment (SoRa)
SRRFing your way to super resolution - with Ricardo Henriques
Elyra 7 with lattice SIM
Isotropic nanometre resolution with minimum photon fluxes (MinFlux)
Stimulated emission depletion (STED)
3D SMLM and Spectral Unmixing
Single molecule localisation with the Vutara