Speakers

Silvio Rizzoli

Georg-August Universität Göttingen

My lab has a dual focus – the development of high-end imaging and synaptic physiology. Our projects combine super-resolution imaging techniques (STED, STORM, expansion microscopy) with conventional imaging, electron microscopy and quantitative biochemistry.

 

We provided the first quantitative molecular description of a part of the cell, the synapse (now included in the standard textbook for cellular and molecular biology, the “Molecular Biology of the Cell” by Alberts and colleagues, Garland Science, sixth edition). We currently extend our studies in the temporal direction by imaging cellular turnover in situ, at the nanoscale, using secondary ion mass spectrometry (SIMS).

 

Finally, we also focus on developing new imaging tools, ranging from new chemical probes to camelid-derived single-chain antibodies (nanobodies), which result in the improved visualization of cellular targets, especially at the nanoscale.

Ilaria Testa

KTH Royal Institute of Technology, Stockholm

I performed my PhD between 2006-2009 at the University of Genoa (Italy) in the group of Prof. A. Diaspro working on the use of Photo-switchable Fluorescent Proteins for cellular trafficking. Between 2009-2014 I worked as a Postdoc Researcher in the group of the Nobel Laureate Prof. S.W. Hell at the MPI-BS in Göttingen (Germany). During this time I actively developed several nanoscopes based on (1) single molecule switching for multicolour imaging (2) RESOLFT nanoscopy. Within an interdisciplinary team of biologists and physicists we succeeded to apply the RESOLFT concept in living cells and even tissues for the first time. Since 2015 I’m Professor at KTH SciLife fellow at the SciLifeLab in Stockholm (Sweden). The goal of my group is to develop next generation fluorescence nanoscopy approaches to address contemporary challenges in biophysics and molecular biology. A special effort will be dedicated to investigate the nanoscale organization of neuronal processes at high spatio/temporal resolution

Andrew York

Calico Labs

I am a physicist with a background in optics, an interest in invention, and no training in biology.

 

My postdoctoral work produced the technology behind super-resolution microscopes like the Yokogawa CSU-W1 SoRa, the VisiTech VT-iSIM, and the Oxford Nanoimager's SIM mode.

 

My group at Calico Labs currently works on microscopy projects related to lightsheet, stimulated emission, deconvolution, photophysics, and fluorophore development. See  our recent publications at andrewgyork.github.io

Chris Peddie

Francis Crick Institute

I am a Principal Laboratory Research Scientist in the Electron Microscopy Science Technology Platform (EM STP) at the Francis Crick Institute in London, where I help to support the electron microscopy needs of the research groups at the institute. 

 

My work focuses mainly on the application of volume electron microscopy techniques in correlating functional data from a variety of light microscopy methods to the underlying cellular structure, and the development of new techniques and technologies within the STP.

 

Specific interests include in-resin fluorescence preservation and the SECOM super-resolution integrated light and scanning electron microscope, and more typical volume electron microscopy applications, including serial blockface and focused ion beam scanning electron microscopy, with extension to the 'smart' microscopy workflows of the future.

Christophe Zimmer

Institut Pasteur

The Imaging and Modeling Unit of Institut Pasteur, develops computational and experimental approaches to characterize and quantitatively predict selected cellular processes.

 

Our current projects concentrate on : (i) investigating the dynamic spatial architecture of the genome and its functional consequences, and (ii) developing high resolution or high throughput imaging techniques, and applying them to study genome architecture and the cell biology of pathogens, especially HIV.

 

Our lab mobilizes a spectrum of expertise including biophysics, microscopy, informatics and cell biology, and works in close collaboration with several experimental groups, many of them at Institut Pasteur.

Sebastian van de Linde

University of Strathclyde

I am currently a Lecturer and Chancellor’s Fellow in the Department of Physics at the University of Strathclyde. In 2011, I obtained a PhD in Physics under the supervision of Markus Sauer at Bielefeld University, Germany, and later worked in the Department of Biotechnology and Biophysics, University of Würzburg, Germany. 

It is already 13 years ago, when we developed dSTORM and started to explore the opportunities of SMLM; since then I worked extensively on the photophysics of organic dyes. 

My group is working on the development of refined single-molecule imaging tools for super-resolution microscopy to study cellular life, including novel photoswitches, optical imaging schemes and software for image processing and data analysis. A special focus of my research interest is the quantitative imaging of membrane receptors. 

Jonas Ries

EMBL - Heidelberg

I  studied physics with a focus on quantum optics in Bremen, Konstanz and Hannover.

During my PhD at the TU Dresden in the group of Petra Schwille, I developed new methods to apply fluorescence correlation spectroscopy (FCS) on membranes.

For my post-doctoral research, I joined the group of Helge Ewers and Vahid Sandoghdar at the ETH Zürich where I started working with super-resolution microscopy.

 

In 2012, I joined EMBL, Heidelberg as a group leader and my group continues with developing novel tools for super-resolution microscopy and with applying them to exciting biological questions.

Susan Cox

King's College London

I am a Royal Society University Research Fellow in the Randall Division of Biophysics at King's College London.

 

Following a PhD in transmission electron microscopy at Cambridge, I spent three years at the National High Magnetic Field Laboratory at Los Alamos looking at the behaviour of the low temperature phases of strongly correlated electron systems.

 

My current primary interest is the development of new super-resolution localisation microscopy techniques, both through the development of optical systems and the creation of novel image analysis algorithms. I use these techniques to investigate the behaviour of the cytoskeleton in live cells at the nanoscale. In 2015, I was awarded the RMS Medal for Light Microscopy and the President's Medal of the Society of Experimental Biology for the Cell Section.

Workshop Academics

Ricardo Henriques

University College London

I have been a group leader since 2013 at both University College London and Francis Crick Institute in the UK.

 

My group undergoes research in optical and computational biophysics, with a special interest in super-resolution microscopy and host-pathogen interactions.

 

 

I graduated in Physics, specialising in biophotonics and robotics. I finished my PhD In 2011 on the topic of advancing super-resolution microscopy technologies (Musa Mhlanga lab). I then pursued postdoctoral research at the Institut Pasteur Paris, studying HIV-1 T-cell infection through nanoscale imaging (Christophe Zimmer lab).

Deirdre Kavanagh

University of Oxford

As an undergraduate at the University of Edinburgh, I studied Medical Microbiology and Infection before completing an interdisciplinary PhD in Electrical Engineering on the subject of Microfluidics for Non-Invasive Prenatal Diagnostics. During my postdocs, I applied advanced fluorescence microscopy and spectroscopy to study the molecular mechanisms underlying cell communication and trafficking.

 

Over the past four years, I have worked as an Imaging Specialist at University of Birmingham, where my primary role was in the teaching and training of researchers in advanced microscopy. In May 2021, I joined the University of Oxford as Microscopy Facility Manager for the Biochemistry Department where I am responsible for the running of a busy microscopy facility and continue my passion for microscopy teaching and training. I have expertise in fluorescence microscopy with specialist interest in super-resolution, FCS and light-sheet technologies.

Industry Partner Workshops

Digital Imaging with low-noise Cameras

Spinning disk super resolution by optical pixel reassignment (SoRa)

SRRFing your way to super resolution - with Ricardo Henriques

Stimulated emission depletion (STED)

Single molecule localisation with the Vutara

Isotropic nanometre resolution with minimum photon fluxes (MinFlux)

Elyra 7 with lattice SIM